Active transport of amino acids in Thiobacillus thioparus is a low-affinity process
نویسندگان
چکیده
منابع مشابه
Transport of amino acids.
Before amino acids nourish the cell they must be transported a) across the intestinal mucosa and b) across the plasma membrane of the cell where they are to be used. In addition, full consideration of their economy in the animal organism must include also their entry into the liver and their resorption from the renal tubule. All of these events appear to have much in common and probably to repr...
متن کاملAssimilation and metabolism of exogenous organic compounds by the strict autotrophs Thiobacillus thioparus and Thiobacillus neapolitanus.
The assimilation and utilization of the individual carbon atoms of pyruvate and acetate by cells of Thiobacillus thioparus and T. neapolitanus, in the presence and absence of an energy source, were studied by use of radioactive substrates. Both organisms produced (14)CO(2) from (14)C-labeled pyruvate, but more came from carbon 1 than from carbons 2 or 3. The conversion of the carbons of acetate...
متن کاملGenetic separation of high- and low-affinity transport systems for branched-chain amino acids in Escherichia coli K-12.
The Escherichia coli K-12 mutant strain AE4107 (livH::Mu) is defective in the high-affinity binding protein-mediated uptake system for L-leucine, L-valine, and L-isoleucine (LIV-I). We have used this strain to produce mutations in the residual LIV-II membrane-bound branched-chain amino acid uptake system. Mutants selected for their inability to utilize exogenous L-leucine were found to be defec...
متن کاملCharacterization of Thiobacillus thioparus LV43 and its distribution in a chemoautotrophically based groundwater ecosystem.
Bacterial strain LV43 was previously isolated from a floating microbial mat located in Movile Cave, the access point to a chemoautotrophically based groundwater ecosystem in southern Romania. This gram-negative, rod-shaped organism grows autotrophically through the oxidation of thiosulfate and sulfide, but it does not grow heterotrophically. Strain LV43 grows over a pH range of 5.0 to 9.0, with...
متن کاملRemoval of hydrogen sulphide by immobilized Thiobacillus thioparus in a biofilter packed with polyurethane foam
In this work, a laboratory scale biofilter was design for removal of hydrogen sulphide packed with Thiobacillus thioparus immobilized in polyurethane foam particles (the initial immobilized biomass was 1.6×10 cel g of solid support). Three empty bed residence times (EBRT) were studied (100, 70 and 30 second) and the initial H2S concentration, ranging from 25 to 750 ppmv, in the inlet gas stream...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1981
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.148.3.956-965.1981